Dilutions made are also instrument-specific, but generally are larger than ones made for manual counts [e.g., TOA Sysmex ä E-5000 makes a 1:750 dilution for a WBC]. Pipettes [Manual Cell Counts] Historically, micropipettes are used to make dilutions of whole blood. One such pipette is the Thoma pipette.
A 1:100 dilution would be 10 mL Surflan AS in 1 L water. 10 mL Surflan contains 1.4 g or 1,400 mg oryzalin. Dissolving 1.4 g oryzalin into 1 L of water would make a 0.004 M solution, which is a 4,000 uM solution. This is just about 23X more concentrated than a 175 uM solution.
Accuracy of serial dilution. To make a 1 /100 dilution of a solution, which, if either, procedure provides more accuracy: transfer 1 mL with a pipet to a 100-mL volumetric flask or transfer 10 mL with a pipet to a 1-L volumetric flask?
1. Weigh out 1 gram of your soil and add this to 99 ml of sterile distilled water (1/100 dilution). Mix well. 2. While still suspended, further dilute the suspension by removing 1 ml with a P-1000 micropipettor and add it to a test tube containing 9 ml of sterile distilled water(1/10 dilution — 1/1000 total dilution). 3.
For example, to make 100 ml of 0.1 M CaCl 2 solution, use the previous formula to find out how much CaCl 2 you need: grams of CaCl 2 = (0.1) x (110.91) x (100) ÷ (1000) = 1.11 g; Now you can make your solution: dissolve 1.11 g of CaCl 2 in sufficient water to make 100 ml of solution. The amount of water needed will be slightly less than 100 ml.
How do you make a 1 100 dilution? For a 1:100 dilution, one part of the solution is mixed with 99 parts new solvent. Mixing 100 µL of a stock solution with 900 µL of water makes a 1:10 dilution. The final volume of the diluted sample is 1000 µL (1 mL), and the concentration is 1/10 that of the original solution.
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